http://www.ebsi.co.kr/ebs/lms/lmsx/retrieveSbjtDtl.ebs?sbjtId=S20180001029 ================================================================================ * Useful DNA like insulin DNA * Cut useful DNA by using restriction enzyme Rrestriction enzyme is discovered in germ Rrestriction enzyme of germ cuts external DNA * Plasmid as carrier DNA * Use "restriction enzyme" on plasmid * Use ligase * Insert "reconstructed plasmid" into E_coli by using electrical stimulus And you have several types E_coli_A: no plasmid E_coli_B: has original plasmid E_coli_C: has reconstructed plasmid * Select "type C E_coli" by using markers ================================================================================ * 4 epitope or antigenic determinant * 4 different antibodies * * Use electrical charge * 4 kinds of mixed cells * separate to each one into 4 cups ================================================================================ PCR (Polymerase Chain Reaction) * When you have too small DNA, you can make many DNA by using PCR * DNA which you want to copy * 2 DNA primers for 2 strands * DNA polymerase * * * * * Step1: increase temperature up to 90C to decouple hydrogen bond * Step2: attach 2 DNA primers onto 2 strands Temperature should be 55C * Synthesize DNA by using DNA polymerase Temperature should be 72C * Get copied DNA * Iterate above step ================================================================================ DNA polymerase in PCR is special DNA polymerase which is obtained from germ which is resistent to the high temperature ================================================================================