https://www.youtube.com/watch?v=XOBCZ2Ht4hQ /mnt/1T-5e7/mycodehtml/Biology/Gene_sequencing/Short_tutorial_text/Personal_Genomics_Institute/SNP_genotyping/main.html ================================================================================ Genotyping - is the thing which determines gene expression ================================================================================ Following differences are made from different genotyping ================================================================================ Why genotyping is different in each person? It's because different SNP ================================================================================ Some person has T, some person has C, some person has G ================================================================================ In entire human genome, there are 10,000,000 SNPs ================================================================================ People have 99.9% of "same characteristic (or same gene)" ================================================================================ But each one has 0.1% of difference (or different gene, or SNP?) ================================================================================ ================================================================================ Why different genetic expression on even sibling? Siblig obtains different SNPs, creating different genetic characteristic But they still have more genetic expression compared to others ================================================================================ If doctor can know the SNPs related to cancer, doctor can perform preventive medicine or better treatments Or, people can have better life style and dietary fit to their SNPs ================================================================================ SNP analyzing methods 1. Axiom method - when customer wants to know over 500000 SNPs - when over-100 number of samples are allowed 2. Fludigm method - when customer wants to know around 50 SNPs - when around 50 number of samples are allowed ================================================================================ You can know SNP information via "decoding DNA sequence" ================================================================================ Genotyping - It only finds partially "interested SNP" from "known DNA sequence" - Checks "disease information along with SNP" ================================================================================ ================================================================================ Genotyping Small sample Short processing time Cheap cost compared to NGS