https://www.youtube.com/watch?v=9qPcYHNL_yg&list=PLaE61CK5r6_l2fxVp3r3OP0fgTSTdQUoQ&index=43&t=0s ================================================================================ - Introduction to Galaxy platform - Get Data: upload data - FastQC: check the "quality of data" and "data format" - FASTQ Groomer: convert "data format" - FASTQ Quality Trimmer: trim the "bad sequence" - FASTQ Filter: remove "bad reads" - How to use Galaxy Workflow ================================================================================ - There are old and new machines - So FASTQ files are slightly different per each machine - Groomer convert raw FASTQ format into latest format - Measure the quality of raw FASTQ data by using FastQC - Bad parts (red areas) can be detected - Trim bad part - If there are too many bad part, remove entire read ================================================================================ - FASTA format - FASTQ format ================================================================================ ================================================================================ ================================================================================ ================================================================================ ================================================================================