https://www.youtube.com/watch?v=B8L2MC0T9zk /mnt/1T-5e7/mycodehtml/Biology/SangSooKim/Bioinformatics_basic/005/002_Transcript_variants_alternative_splicing/main.html ================================================================================ Copy reference_sequence_of_mRNA and search it ================================================================================ In Nucleotide database, paste above reference_sequence_of_mRNA ================================================================================ ================================================================================ This transcript variant 2 is a bit short than transcript variant 1 (8460 bp) ================================================================================ - CDS ================================================================================ 8427 |127-1620|=1494, size of coding-sequence transcript variant 1 |160-1653|=1494, size of coding-sequence ================================================================================ Click CDS ================================================================================ - taa: terminating codon ================================================================================ Back to the first page - 608th base pair in coding sequence ================================================================================ 608 (meaning 608th base pair in coding sequence) + 127 - 1 = 734 734th base pair is variant in terms of entire mRNA sequence ================================================================================ - Let's find following codons ================================================================================ ================================================================================ 2 NMs (mRNA) creates 2 NPs (proteins), by using translation - mRNAs - Proteins ================================================================================ - mRNA - protein ================================================================================ - gene which is composed of 4 axons? - Splicing: introns are removed, axons are gathered ================================================================================ - After axons gathers, "alternative splicing" happens, creating "spliced form (or isoform)" ================================================================================ - Case1: red axon is removed - Case2: all axons are used - Case3: green axon is removed - There are much more variations ================================================================================