https://www.youtube.com/watch?v=R-VJkyl8PeY /mnt/1T-5e7/mycodehtml/Biology/SangSooKim/Bioinformatics_basic/005/003_Gene_database/main.html ================================================================================ Gene XIAP has 2 mRNAs as alternative splicing - How can you know "gene XIAP has 2 mRNAs"? - In Nucleotide database, search XIAP ================================================================================ ================================================================================ Gene XIAP is in various living creatures Human being Mouse Chimpanzee ================================================================================ Click ================================================================================ ================================================================================ Click RefSeq: - Many researchers upload their result sequence onto the corresponding database - NCBI removes duplicated results - And NCBI creates RefSeq which contains no duplicated data ================================================================================ - mRNA_1 for XIAP gene - mRNA_2 for XIAP gene ================================================================================ - non-coding RNA click ================================================================================ - Gene: XIAP - Alternative splicing: transcription variant 3 - Axon/intron: intron (non-coding RNA) ================================================================================ - NG: DNA sequence (axon+intron) - much more longer Click ================================================================================ ================================================================================ There is one more XM as alternative splicing ================================================================================ - NM: mature mRNA - XM: predicted model-mRNA which is not proved in experiments ================================================================================ - Transcription on XM, and get predicted model-protein-products ================================================================================ - If you search "XIAP" and "Human" and "mRNA", click ================================================================================ - Be careful on "associated factors" - These are not XIAP, but XAF1 gene which is related to XIAP ================================================================================ Another way to get same result ================================================================================ ================================================================================ ================================================================================ This is translated into ================================================================================ ================================================================================ - Researchers confirmed the result ================================================================================ ================================================================================ Click ================================================================================ ================================================================================