https://www.youtube.com/watch?v=pQkbSGLLT6o /mnt/1T-5e7/mycodehtml/Biology/SangSooKim/Bioinformatics_basic/006/002_Translation_for_protein_ORF_finder/main.html ================================================================================ Open Reading Frame estimated_sequence=sequencing(mRNA) which part is for protein?: find(estimated_sequence) ================================================================================ Case1: when you know translation direction: out_list=run(3_possible_cases) Case2: when you don't know translation direction: out_list=run(6_possible_cases) ================================================================================ ORF=get_parts_for_protein(out_list) ================================================================================ Possible cases: 6 when you don't know translation direction ================================================================================ 5' ---> 3' 3' <--- 5' ================================================================================ Translation_candiate_1 Y (Tyrosine) TAT S (Serine) TCC * (Termination codon) TGA /home/young/Pictures/2019_07_01_12:53:13.png ================================================================================ Translation_candiate_2 I (Isoleusin) ATT P (Proline) CCA I (Isoleusin) ATT /home/young/Pictures/2019_07_01_12:55:17.png ================================================================================ Translation_candiate_3 F (Phenylalanine) TTC /home/young/Pictures/2019_07_01_12:56:28.png ================================================================================ ORF finder from NCBI, which finds best "Open Reading Frame" ================================================================================ - 2nd and 5th ones look good as "true OFR" (long green means long protein) ================================================================================ ================================================================================ Click FASTA to see only "sequence part" FASTA: sequence alignment program ================================================================================ - Description in one line ================================================================================ Copy all including description ================================================================================ ================================================================================ ================================================================================ Or you can go to ORF finding directly by searching "ORF finder" ================================================================================ ================================================================================ Click "OrfFind" ================================================================================ ================================================================================ ================================================================================